Effects of pilose antler polypeptide on abilities of proliferation and colagen secretion of mouse embryonic fibroblasts NIH/3T3 and ther mechansms / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the effects of pilose antler polypeptide on the abilities of proliferation and collagen secretion of mouse embryonic fibroblasts NIH/3T3, and to clarify the relevant mechanisms. Methods: The NIH/3T3 cells were treated with different doses 1.56, 3.13, 6.25, 12.50, 25.00, 50.00, 100.00, and 200.00 mg • L_ 1) of pilose antler polypeptide as experimental groups, the cells treated with 0 mg • L_ 1 pilose antler polypeptide were used as blank control group, and the cells treated with 50.00 fig • L-1 basic fibroblast growth factor (bFGF) were used as positive control group. MTT assay was used to detect the survival rates of NIH/3T3 cells in various groups. ELISA assay was used to detect the collagen secretion of NIH/3T3 cells in various groups. Wound healing assay was used to detect the migration abilities of NIH/3T3 cells. Western blotting method was performed to detect the expression levels of p-ERK 1/2 in the NIH/3T3 cells in various groups. Immunofluorescence method was used to detect the expression levels of transforming growth factor-fil (TGF-J31) in the NIH/3T3 cells in various groups. Results: Compared with blank control group, the survival rates of NIH/3T3 cells in positive control group and 6.25, 12.50, 25.00, 50.00, 100.00, 200.00 mg • L_ 1 pilose antler polypeptide groups were markedly increased (P < 0 . 05 or P < 0 . 01). Compared with blank control group, the levels of type I collagen protein in the culture solution of the NIH/3T3 cells in positive control group and 6. 25, 12. 50, 25. 00, and 50. 00 mg • L_ 1 pilose antler polypeptide groups were markedly increased (P < 0 . 05 or P < 0 . 01), and the levels of type IE collagen protein in the culture solution of the NIH/3T3 cells in positive control group and 12. 50 and 25.00 mg • L_ 1 pilose antler polypeptide groups were markedly increased (P < 0 . 05). Compared with blank control group, the scratch healing rates of NIH/3T3 cells, and the expression levels of p-ERK 1/2 in the NIH/3T3 cells, and the expression levels of TGF-J31 in the NIH/3T3 cells in positive control group and 12. 50 mg • L_ 1 pilose antler polypeptide groups were markedly increased (P < 0 . 05 or P < 0 . 01). Conclusion: Pilose antler polypeptide can promote the proliferation, and collagen secretion of NIH/3T3 cells and increase the migration ability, which may be achieved by activating the phosphorylation of ERK 1/2 and increasing the expression of TGF-J31.