Preparation process of polylactic acid-glycolic acid copolymer/hydroxyapatite microcarrier by electrostatic spraying method and its effect evaluation / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the preparation process of polylactic acid-glycolic acid copolymer/ hydroxyapatite (PLGA/HA) microcarriers by electrostatic spraying method, and to elucidate the superiority of PLGA/HA microcarriers. Methods: The PLGA/HA microcarriers were prepared by electrostatic spraying method using nano-HA (20 nm, 99.9%) and PLGA (LA/GA = 50/50, Mw30k), and the influence of different concentrations (1%, 3%, 5%) and different voltages (4. 0, 4. 5, > 5. 0 kV) of HA in the morphology of the microcarriers was investigated and the best ball making parameters were obtained. The PLGA microcarriers were used as PLGA group, PLGA+1%HA as 1% PLGA/HA group, PLGA + 3% HA as 3% PLGA/HA group, PLGA+ 5% HA as 5% PLGA/HA group, and the simple cells were used as blank control group. The characteristics of PLGA/HA microcarriers were detected by scanning electron microscope (SEM), cell proliferation test, cell fluorescence staining experiment, and Fourier transform infrared spectroscopy (FTIR). Results: The SEM results showed that the microcarrier particles were uniform, all of them were elliptical or circular, without abnormal shape spheres, with smooth surface, without sharp edges, adhesion between the spheres and aggregation, and there were no significant differences between the different concentrations of microcarriers. The cell proliferation test results showed that the order of adhesion cells was 5% PLGA/HA group > 3% PLGA/HA group > 1% PLGA/HA group > PLGA group > blank control group (P<0. 05); the number of cells was increased with the increasing of HA concentration; the microcarriers in 5% PLGA/HA group had the best cell affinity and the microcarriers had no cytotoxity. The cell fluorescence staining experiment showed that the MC3T3-E1 cells adhered well on the microcarriers. The FTIR analysis results showed that HA characteristic absorption peak was observed, indicating that the composite microcarrier contained PLGA and HA. Conclusion: The preparation process of PLGA/ HA microcarriers is successfully established by electrostatic spraying method. The method is simple and convenient to operate, and has excellent ball-making effect. It has broadly application prospects in bone tissue engineering.