Inhibitory effect of 18β-glycyrrhetinic acid on inflammation-related gastric cancer and its mechanism / 吉林大学学报(医学版)

ABSTRACT

Objective: To explore the inhibitory effect of 18β-glycyrrhetinic acid (18β-GA) on the inflammationrelated gastric cancer, and to clarify its mechanism. Methods: A total of 72 K19-Wnt/C2mE transgenic mice with gastric cancer were randomly divided into 18β-GA treatment group (drinking water contain 0.1 % 18β-GA, n-36) and control group (drinking normal water, n-36). After 52 weeks, the incidence of gastric cancer and morphology of gastric mucosa of the mice in two groups were detected. Immunohistochemistry staining was used to detect the histochemistry scores (H-score) of Ki-67, F4/80, ATP4a, KCNE2, pepsinogen C (PGC), Wnt-1, β-catenin, and cyclooxygenase-2 (COX-2) in gastric mucosa epithelial cells of the mice in two groups. Results: The gastric mucosa of the mice in control group showed protruded lesions, atypical hyperplasia and chronic gastritis. Compared with control group, the incidence (P-0. 019) and the volume of gastric cancer of the mice in 18β-GA treatment group were significantly decreased (P<0. 01); the structural heterozygosities of gastric cells and tissue were decreased, and the inflammation reactions of the mice in 18β-GA treatment group were alleviated. Compared with control group, the H-scores of Ki-67, F4/80, Wnt-1, β-catenin, and COX-2 in gastric mucosa epithelial cells of the mice in 18β-GA treatment group were decreased (P<0.05), and the H-scores of ATP4a, KCNE2, and PGC were increased (P<0. 05). Conclusion: 18β-GA can significantly inhibit the occurrence of gastric cancer in the K19-Wnt/C2mE transgenic mice. The inhibitory effect may be related to alleviating the inflammation reaction and promoting the differentiation of gastric mucosa epithelial cells and inhibiting the occurrence of gastric cancer.