Effects of miR-26a targeting HMGA1 gene on growth, invasion and migration of colon cancer cells / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the effects of miR-26a targeting high mobility group protein 1 (HMGA1) gene on the growth, invasion and migration of colon cancer cells, and to clarify whether the HMGA1 gene was the target gene of miR-26a. Methods: The miR-NC (miR-NC group), miR-26a mimics (miR-26a mimics group) and miR-26a inhibitor (miR-26a inhibitor group) were transfected into the human colon cancer SW480 cells. RT-PCR and Western blotting methods were used to detect the mRNA and protein expression levels of HMGA1. Luciferase reporter gene detection kit was used to detect the double luciferase activity in SW480 cells and to determine whether HMGA1 was the target gene of miR-26a. The cell proliferation activities of cells in various groups were detected by CCK8 assay; the number of invasion and migration cells was detected by Transwell chamber method. Results: HMGA1 gene was the target gene of miR-26a. Compared with miR-NC group, the proliferation activities of colon cancer SW480 cells in miR-26a mimics group at after 24, 48 and 72 h after transfection were significantly decreased (P<0. 01), while the proliferation activities of colon cancer SW480 cells in miR-26a inhibitor group were significantly increased (P<0. 01). Compared with miR-NC group, the proliferation activities of colon cancer SW480 cells, the number of invasion and migration cells in miR-26a mimics group and miR-26a mimics + pcDNA3. 1-HMGA1 group at different time points were significantly decreased (P<0. 01); the proliferation activities of colon cancer SW480 cells, the number of invasion and migration cells in miR-NC + pcDNA3. 1-HMGA1 group were increased (P<0. 01). Compared with miR-26a mimics group, the proliferation activities of colon cancer SW480 cells, the number of invasion and migration cells in miR-26a mimics+ pcDNA3. 1-HMGA1 group at different time points were increased (P<0. 01); compared with miR-NC+pcDNA3. 1-HMGA1 group, the proliferation activities of colon cancer SW480 cells, the number of invasion and migration cells in miR-26 mimics + pcDNA3. 1-HMGA1 group at different time points were decreased (P<0. 01). Conclusion: HMGA1 gene is the target gene of miR-26a. Up-regulation of the expression of miR-26a can inhibit the proliferation of colon cancer SW480 cells and downregulation of the expression of miR-26a can promote the proliferation of colon cancer SW480 cells.