Inhibitory effect of resveratrol on pulmonary inflammation in mice with neutrophilic asth ma and its mechanism / 吉林大学学报(医学版)

ABSTRACT

Objective; To investigate the inhibitory effect of resveratrol on the pulmonary inflammation in the mice with neutrophilic asthma, and to elucidate its mechanism. Methods; A total of 18 female C57BL/6J mice were selected. The mouse models of neutrophilic asthma were established by sensitization with lipopolysaccharide (LPS) and ovalbumin (OVA). The model mice were randomly divided into model group (LPS + OVA group), resveratrol group (Res group, intragastric administration of 30 mg. kg-1 resveratrol at 2 h before challenge) and N-acetylcysteine group (NAC group, intragastric administration of 3 mmol · kg-1 N-acetylcysteine at 2 h before challenge). Other 6 age-matched female C57BL/6J mice were choosen as normal control group (PBS group). The behavioral changes of mice in various groups were observed during the atomization with methacholine; the airway hyperresponsiveness (AHR) of mice was analyzed with a lung function instrument, and the total number of cells and inflammatory cells in the bronchoalveolar lavage fluid (BALF) were observed with light microscope; the pathological changes of lung tissue were observe by HE staining; the levels of interleukin-17 (IL-17) in the supernatant of BALF of the mice in various groups were detected by enzyme-linked immunosorbent assay (ELISA); the level of reactive oxygen species (ROS) in lung tissue was analyzed by specific fluorescent probe DCF-DA staining; the level of malondialdehyde (MDA) in lung homogenate was measured by MDA kit. Results: Compared with PBS group, the Penh value, total number of cells, inflammatory cells, airway inflammation and scores of the mice in LPS+OVA group were significantly increased (P<0. 05 or P<0. 01); the IL-17 level in BALF supernatant was significantly increased (P<0. 01); the ROS fluorescence intensity in lung tissue was significantly increased, and the MDA level in lung homogenates was significantly increased (P<0. 01). Compared with LPS + OVA group, the Penh value, total number of cells, inflammatory cells, airway inflammation and scores in Res group and NAC group were significantly decreased (P<0. 05 or P<0. 01); the IL-17 levels in BALF supernatant were significantly decreased (P<0. 05 or P<0. 01); the ROS fluorescence intensities in lung tissue were decreased, and the MDA levels in lung homogenates were significantly decreased (P < 0.01). Conclusion: Resveratrol can effectively inhibit the inflammatory response of lung tissue in the mice with neutrophilic asthma, and its mechanism may be related to inhibiting the oxidative stress reaction.