Changes of levels of IL-7 and IL-10 in peripheral blood of patients with different clinical stages of eczema and their clinical significances / 吉林大学学报(医学版)

ABSTRACT

Objective: To detect the levels of interleukin-7 (IL-7) and IL-10 in peripheral blood of the patients with different clinical stages of eczama, and to elucidate their clinical significances. Methods: Ninety patients with eczema were selected, and divided into acute eczama group (n=30), subacute ecxama group (n=30), and chronic eczama group (n=30). Meanwhile, 30 healthy people who went to hospital for physical examination during the same period were recruited as control group. The relative expression levels of cytokines IL-7 and IL-10 mRNA in peripheral blood of the subjects in various groups were detected by RT-PCR method. The levels of IL-7 and IL-10 in peripheral blood of the subjects in various groups were measured by ELISA method. The eczema area and severity index (EASI) scores of the subjects in various groups were detected. The relationships between IL-7 level, IL-10 level and EASI scores were analyzed. Results: Compared with control group, the relative expression levels IL-7 and IL-10 mRNA in peripheral blood of the patients with different clinical stages of eczenma were significantly increased (F= 3. 17, P= 0. 04; F= 4. 73, P = 0. 02); the expression levels of IL-7 and IL-10 in peripheral blood of the patients with different clinical stages of eczema were significantly increased (F= 6. 23, P<0. 01; F= 5. 34, P= 0. 01). The levels of IL-7 and IL-10 were positively correlated with the corresponding mRNA relative expression levels in peripheral blood of the patients with different clinical stages of eczema (r= 0. 95, P<0. 01; r= 0. 94, P<0. 01) and EASI scores of the patients (r=0. 88, P<0. 01; r= 0. 89, P<0. 01), which were increased along with the increasing of severity degree of the patients. Conclusion: The levels of IL-7 and IL-10 of the patients with eczema are significantly higher than those in the healthy controls, which are positively correlated with the severity of the disease. The cytokines IL-7 and IL-10 may be involved in the pathological process of eczema, and the detection of their experssion levels contributes to assess the severity of disease.