Preparation and application of rabbit anti-osteoclastic protein tyrosine phosphatase polyclonal antibody / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition)
;
(6): 661-666, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-841906
ABSTRACT
Objective:
To purify the recombinant protein by using the constructed pET28a-APTP-oc recombinant expression plasmid, and to prepare the rabbit polyclonal antibody against osteoclastic protein tyrosine phosphatase (PTP-oc) and to identify its properties.Methods:
The constructed pET28a-APTP-oc recombinant plasmid vyas transformed into E.CO BL21 (DE3), and the isopropyl-ß-D-thiogalactoside (IPTG) vyas used to induce the expression of recombinant protein. The recombinant protein vyas purified by the method of Q fast flovy agarose and Ni-NTA agarose, and the purified recombinant protein vyas used as an antigen to immunize the rabbit to obtain PTP-oc polyclonal antibody. The titer and specificity of the antibody vyere identified by indirect ELISA and Western blotting methods.Results:
The recombinant PTP-oc protein vyas successfully purified. The indirect ELISA results shovyed that after immunizing the rabbit vyith the recombinant PTP-oc protein, the polyclonal antibody vyith the titer of 1 3 2000 vyas gotten. The Western blotting results shovyed that the polyclonal antibody had high specificity.Conclusion:
The PTP-oc recombinant protein is successfully purified, and the polyclonal antibody of PTP-oc is prepared successfully.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Jilin University(Medicine Edition)
Year:
2018
Type:
Article
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