Effects of Xiaoaiping combined with X-rays radiation on proliferation and apoptosis of human liver cancer HepG2 cells / 吉林大学学报(医学版)

ABSTRACT

Objective: To detect the changes of the proliferation, cell cycle and apotopsis of liver cancer HepG2 cells after treated with Xiaoaiping (XAP) combined with X-rays radiation, and to clarify their anti-tumor mechanisms. Methods: The human liver cancer HepG2 cells at logarithmic phase were selected, and divided into control, XAP, 2 Gy radiation and XAP + 2 Gy radiation groups. The HepG2 cells were treated with 75 mg · L-1 final concentration of XAP, and they were irradiated with 2 Gy X-rays 12 h later. The cell proliferation activity was detected with CCK8 kits, the cell cycle and apoptotic rates were measured by FCM with PI single staining and Annexin V-FITC double staining, and the caspase-3 protein expression was measured by Western blotting method. Results: After the cells were treated with XAP, the proliferation activities in XAP, 2 Gy radiation and XAP+2 Gy radiation groups were significantly decreased compared with control group at 12, 24 and 48 h (P<0. 05 or P< 0. 01); the percentages of cells at S and G2/M phases in XAP, 2 Gy radiation and XAP + 2 Gy radiatin groups were significantly increased compared with control group (P<0. 05 or P<0. 01); the apoptotic rates were significantly increased compared with control group (P<0. 05 or P<0. 01), especially in XAP + 2 Gy radiation group. The Western blotting results showed that caspase-3 was cleaved into 17 000 and 19 000 fragments, and its expression was also increased compared with control group. Conclusion: XAP combined with X-rays radiation could inhibit the proliferation, and induce the G2/M arrest and the apoptosis of liver cancer HepG2 cells; the combination of them shows a synergistic anti-tumor effect.