Protective effect of Angelica polysaccharide on retinal nerve cells in glaucoma rats and its mechanism / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the protective effect of Angelica polysaccharide on the glaucoma-induced retinal neuronal injury, and to elucidate its mechanism. Methods: Eighty-four SD rats were randomly divided into control group, model group, positive drug group, low, middle and high doses of Angelica polysaccharide groups. The model of rat glaucoma was established by sclera sclerotherapy. The intraocular pressures (IOP) of rats in each group were measured. The superoxide dismutase (SOD) activities, malondialdehyde (MDA) contents and nitric oxide (NO) levels in retina tissue of the rats were measured by colorimetric assay. The expression levels of Caspase-3 mRNA and protein in retina tissue of the rats were observed by RT-PCR and Western blotting method. Results: Compared with control group, the IOP of the rats in model group at each time point were significantly increased (P<0. 05); compared with model group, the IOP of the rats in different doses of Angelia polysaccharide groups were significantly decreased (P<0. 05); compared with low dose of Angelica polysaccharide group, the IOP of the rats in high dose of Angelica polysaccharide group was significant decteased (P<0. 05). The levels of MDA and NO in the retina tissue of the rats in model group were higher than those in control group (P<0. 05), but the SOD activities were decreased (P<0. 05); compared with model group, the levels of MDA and NO in retinal tissue of the rats in different doses of Angelica polysaccharide group were significantly decreased (P<0. 05), and the SOD activities were significantly increased (P<0. 05); compared with low dose of Angelica polysaccharide group, the above indexed had significant differences in high dose of Angelica polysaccharide group (P<0. 05). Compared with model group, the expression levels of Caspase-3 mRNA and protein in different doses of Angelica polysaccharide groups were significantly decreased (P<0. 05); compared with low dose of Angelica polysaccharide group, the expression levels of Caspase-3 mRNA and protein were significantly decreased (P < 0. 05). Conclusion: Angelica polysaccharide has protective effect on the retinal tissue of glaucoma model rats, which can decrease the levels of MDA and NO in retina tissue and increase the activity of SOD and decrease the expression levels of Caspase-3 mRNA and protein in retina tissue. It may be one of the mechanisms of polysaccharide protecting in retinal tissue nerve cells.