Simultaneous Determination of Eight Active Components in Liuwei Wuling Tablet Using HPLC / 中草药·英文版
Chinese Herbal Medicines
;
(4): 331-336, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-842215
ABSTRACT
Objective To develop an effective method for simultaneous determination of eight major components in Liuwei Wuling Tablet (LWT). Methods Reversed phase HPLC method was used with variously improved conditions. Results Salidroside, forsythoside A, specnuezhenide, phillyrin, schisandrol A, schizandrol A, schizandrin A, and schizandrin B in LWT were successfully separated on a Kromasil 100-5-C18 reverse phase column (250 mm × 4.6 mm, 5 μm) using acetonitrile-0.1% phosphoric acid (gradient elution) as mobile phase. The detection wavelength was 275 nm with flow rate of 1.0 mL/min, and the column temperature was maintained at 35 oC. The recovery rate of the method was within the range of 95.13%–104.56% and the precision (RSD) was less than 3% for all eight analytes. All the compounds showed good linearities (r2 > 0.9980) in a relatively wide concentration range. Conclusion Simultaneous quantification of the multiple components by HPLC would be a better strategy for the quality evaluation of LWT.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Herbal Medicines
Year:
2016
Type:
Article
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