Identification of Icaritin Metabolites in Rats by LC-MS/MS / 中草药·英文版
Chinese Herbal Medicines
;
(4): 296-302, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-842299
ABSTRACT
Objective:
Icaritin is the main aglycone and also active intestinal metabolite of prenylflavonoids from the Chinese materia medica Epimedii Herba. Modern pharmacological studies have demonstrated that icaritin has a wide range of biological activities. However, its metabolites and biotransformation pathways have not yet been comprehensively investigated. The present study aims to identify icaritin metabolites in rats by using a sensitive and effective LC-MS/MS method.Methods:
The plasma and urine samples of rats were collected before (blank) and after oral administration of icaritin, and subjected to liquid-liquid extraction with ethyl acetate. The full-scan LC-MS chromatograms of the plasma and urine samples were compared with those of blank samples to detect the possible metabolites, which were later detected by their product ion spectra.Results:
A total of 23 metabolites were identified, and conjugated icaritins produced by glucuronidation, glycosylation, and sulfation were its major metabolites. Minor demethylation, hydrogenation, and oxidation metabolites were also found.Conclusion:
Phase II metabolism is the main metabolic pathway of icaritin.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Herbal Medicines
Year:
2015
Type:
Article
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