Regeneration of Carthamus tinctorius from Jimsar / 中草药·英文版

ABSTRACT

Objective: To investigate the factors significantly affecting the shoot buds regeneration of Carthamus tinctorius from Jimsar (CTJ). Methods: Through tissue culture experiments, the influences of culture temperature, illuminance, relative humidity, explants type, seedlings age, and plant growth regulators added in culture media on in vitro regeneration of CTJ were investigated. Results: The culture temperature was set at 24 °C in daytime and 16 °C at night, illuminance at 9000 lx, and 60% relative humidity were suitable for CTJ regeneration. Cotyledons excised from 6-8 d old seedlings were more responsive than even-aged euphyll, hypocotyl, and root explants because of inducing adventitious buds. The highest percentage of regenerated shoots (79.1%) with about five adventitious buds per responding explant was obtained from MS basal medium containing 12.0 mg/L TDZ, 2.5 mg/L IBA, and 1.5 mg/L 2-ip (No. 14 medium). Regenerated shoot buds (80%) could elongate successfully after 1-2 weeks transferred to shoot elongation medium. Conclusion: Suitable factors of shoot buds regeneration for CTJ were determined. It may also make a useful reference for regeneration researches of other C. tinctorius varieties in China. © 2014 Tianjin Press of Chinese Herbal Medicines.