Metabolomics study of the effect of arsenic trioxide on hepatocellular carcinoma cell line HepG2 / 上海交通大学学报(医学版)
Journal of Shanghai Jiaotong University(Medical Science)
;
(12): 1145-1151, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-843580
ABSTRACT
Objective:
To reveal the mechanism of the effect of arsenic trioxide (ATO) on hepatocellular carcinoma (HCC) cells through metabolomics study.Methods:
HCC cell line HepG2 was treated with ATO for 12 h, 24 h and 36 h respectively. The global metabolite profiles were monitored by metabolomics analysis using GC/MS and LC/MS/MS.Results:
A total of 307 certified metabolites were detected. Bioinformatics analysis showed that glycosylation and one-carbon metabolism were the main cellular functions that have been affected. Other affected cellular functions/pathways included mitochondrial function fatty acid β-oxidation, TCA cycle, pentose phosphate pathway (PPP) and glutathione metabolism.Conclusion:
ATO treatment affects the main metabolomic pathway of HepG2, and inhibits cell growth and migration, then exacerbates oxidative stress to induce cancer cell apoptosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Shanghai Jiaotong University(Medical Science)
Year:
2018
Type:
Article
Similar
MEDLINE
...
LILACS
LIS