Effect of resveratrol on proliferation of CT26 colonic cancer cells / 上海交通大学学报（医学版）

ABSTRACT

Objective: To investigate the effect of resveratrol (RSV) on proliferation of mouse colonic cancer cells CT26 and its underlying molecular mechanisms. Methods: CT26 cells were incubated with 20, 40, 60, 80 and 100 μmol/L RSV respectively for 24 h. The effect of RSV on cell proliferation was tested with CCK8 kits. The effect of RSV on cell apoptosis was detected by living cell dye Hoechst 33342. The plasma protein and nucleoprotein of the cells were extracted separately by using the nuclear protein and cell plasma protein extraction kits. And the expressions of apoptosis related proteins (Bax and Bcl-2), PTEN, NF-κB and Akt were detected by Western blotting. Results: RSV inhibited the proliferation of CT26 cells. The inhibitory effect was dose-dependent. Treatment with RSV promoted the apoptosis of CT26 cells. The expression of NF-κB protein in the cell nucleus was downregulated compared to the control cells, and the expression of NF-κB in the cytoplasm was up-regulated in the CT26 cells after 24 h incubation with RSV. In the CT26 cells after 24 h incubation of RSV, the expressions of Bax and PTEN were up-regulated, the expression of Bcl-2 was down-regulated, and the phosphorylation of Akt protein was down-regulated. Conclusion: RSV can inhibit the proliferation of colon cancer cells in mice, which may be associated with Bax, Bcl-2, NF-κB, p-Akt and PTEN.