Comparison of ex vivo lung perfusion with cold storage in rat donor lung preservation / 上海交通大学学报（医学版）

ABSTRACT

Objective: To establish stable rat ex vivo lung perfusion (EVLP) model and compare the effect of preservation between EVLP group and cold storage group. Methods: Eight SD rats were reconditioned with EVLP for 4 h and 8 SD rats were under 3-h cold storage and 1-h EVLP. The biological index, wet-to-dry weight ratio and concentration of Evans blue were analyzed between two groups. Results: Wet-to-dry weight ratio of lung tissue in EVLP group was significantly lower (5.08±0.88 vs 6.09±0.48, P=0.012); Oxygenation index in EVLP group was significantly higher [(337.9±35.5) mmHg vs (300.5±21.6) mmHg, P=0.023]; Concentration of Evans blue in EVLP group was significantly lower [(36.5±20.3) μg/mL vs (65.0±29.9) μg/mL, P=0.043]. Conclusion: EVLP as an alternative to traditional cold storage technique can effectively alleviate the lung damage.