Notch1 mediates breast cancer epithelial-mesenchymal transition through Slug / 西安交通大学学报(医学版)

ABSTRACT

Objective: To investigate the mechanism of Nocth1 in regulating epithelial-mesenchymal transition (EMT) in breast cancer. Methods: Jagged1 or shRNA was applied to regulate Notch signaling expression. EMT process and migration and invasion in breast cancer were determined. Moreover, Slug luciferase reporter assay was used to investigate the effect of Notch1 on Slug expression. And Slug expression was regulated by shRNA. The effects of Slug on Notch1 mediated breast cancer EMT process and migration and invasion were determined. Results: Jagged1 activated Notch signaling and promoted EMT process and migration and invasion in breast cancer. Overexpression of Notch1 could induce Slug promoter activation, and Slug overexpression could reverse the inhibition of Notch1 shRNA on breast cancer's EMT process and migration and invasion. Conclusion: Notch1 regulates EMT process, migration and invasion in breast cancer through targeting the Slug promoter.