The role of MDM2/p53 pathway in regulating apoptosis of multiple myeloma RPMI8226 cells / 西安交通大学学报(医学版)

ABSTRACT

Objective:To study the regulatory effect and mechanism of MDM2/p53 pathway on apoptosis in multiple myeloma (MM). Methods: RPMI8226 cells were cultured in vitro and divided into Nutlin-3a group, control group and blank group. CCK-8 method was used to detect the cell inhibition rate, Western blot was used to detect the expression of apoptosis-related protein, and flow cytometry was used to detect the apoptosis in the two groups. Results: Compared with that in control group, the inhibition rate of cells in the experimental group increased significantly at 24 h, 48 h and 72 h (P<0.05). Compared with those in the control group, the expression levels of MDM2 and Bcl-2 in the experimental group decreased significantly (P<0.05), while the expression level of p53 increased significantly (P<0.05). The apoptosis rate was significantly higher in the experimental group than in the control group at 24 h, 48 h and 72 h (38.42%, 82.26% and 82.74% vs. 4.80%, 8.06% and 14.69%). Conclusion: The degradation trans-activation pathway between MDM2 and p53 affects the expression of Bcl-2, inhibits the proliferation of MM cells, and promotes the apoptosis of MM cells.