Effects of astragalus polysaccharide on radiation-induced adipogenic differentiation of bonemarrow mesenchymal stem cells / 西安交通大学学报(医学版)
Journal of Xi'an Jiaotong University(Medical Sciences)
;
(6): 304-308, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-843913
ABSTRACT
Objective:
To study the effects of astragalus polysaccharide (APS) on adipogenic differentiation of human bone marrow mesenchymal stem cells (BMSCs) irradiated by X-ray.Methods:
Human bone marrow mesenchymal stem cells irradiated by 2 Gy X-ray were intervened with 50 μg/mL of APS. The number and size of lipid droplets of stem cells were observed by oil red O staining and the expressions of CEBPα and PPAR-γ were detected by Western blot after induction with special medium.Results:
The number of adipocytes differentiated from bone marrow mesenchymal stem cells decreased, and the area of lipid droplets in adipocytes decreased significantly after irradiation (P<0.05). The area of lipid droplets in the bone marrow mesenchymal stem cells treated with APS in advance increased compared with that in radiation alone (P<0.05). Similarly, the expressions of PPAR-γ and recombinant human CCAAT enhancer binding protein (CEBPα ) decreased after X-ray irradiation, while the expressions of PPAR-γ and CEBPα increased after the intervention of APS (P<0.05).Conclusion:
X-ray can damage the directional adipogenic differentiation of human bone marrow mesenchymal stem cells, and APS has a protective effect on the adipogenic differentiation of human bone marrow mesenchymal stem cells after X-ray irradiation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Xi'an Jiaotong University(Medical Sciences)
Year:
2020
Type:
Article
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