Effect of aldehyde scavenger-salicylamine on atherosclerosis of uremic ApoE-/- mice / 西安交通大学学报(医学版)

ABSTRACT

Objective: To observe the effect of aldehyde scavenger, salicylamine (SAM), on atherosclerosis (AS) and its phenotype in uremic apolipoprotein E knockout (ApoE-/-) mice. Methods:Uremic ApoE-/- mice model was created by 5/6 nephrectomy; control ApoE-/- mice were sham-operated. Three subgroups of experimental mice were set up uremia SAM intervention group, uremia group and control group, which were treated with SAM (1 g/L) or vehicle for 6 weeks, respectively. After the intervention was completed, we assessed the body weight, blood pressure, renal function, serum lipid profile, serum SAM concentration, extent and characteristic of aortic atherosclerotic lesion in each group of mice. Results: Compared with control group aortic AS lesion area, necrotic area and macrophage content in AS lesion increased but collagen content in AS lesion decreased in uremia group. SAM treatment for 6 weeks lessened the atherosclerotic lesion area, necrotic area and macrophage content of plaques, and meanwhile increased collagen content of plaques in uremic mice, not accompanied by changes in body weight, blood pressure, serum lipid profile or renal function. SAM did not accumulate or induce toxic effect on uremic ApoE-/- mice. Conclusion: Aldehyde scavenger SAM ameliorates renal injury-induced acceleration of AS, alters atherosclerotic phenotype, and increases the stability of plaques. These benefits are independent of effects on blood pressure, lipid profile or renal function. SAM does not accumulate or induce toxic effect in uremic ApoE-/- mice.