miR-3127-5p promotes hepatocellular carcinoma cell migration and invasion through targeting Cdk10 / 西安交通大学学报(医学版)

ABSTRACT

Objective: To investigate the expression level, clinical significance and mechanisms of miR-3127-5p in hepatocellular carcinoma (HCC). Methods:Real-time quantitative PCR (qPCR) was performed to detect miR-3127-5p expression in HCC tissues and the adjacent non-tumor tissues as well as in HCC cell lines and normal hepatic cells. The difference in miR-3127-5p expresssion between normal liver tissues and HCC tissues was analyzed by TCGA database. The relationship of miR-3127-5p expression with the clinicopathologic features and prognosis of HCC patients was analyzed. After the HCCLM3 cells were transfected with miR-3127-5p inhibitors or negative control sequence, the abilities of cell migration and invasion were measured by Transwell assay; the potential downstream of miR-3127-5p was predicted by TargetScan and validated by Luciferase reporter assay. Results: Compared with that in tumor adjacent tissues or normal hepatic cells, miR-3127-5p was increased in HCC tissue and cell lines (all P<0.05). The expression of miR-3127-5p was significantly related to the portal vein tumor thrombus (P=0.016) and advanced TNM stage (P=0.016). The result of overall survival investigated by TCGA database showed that the survival rate in patients with high miR-3127-5p expression was significantly lower than that in those with low miR-3127-5p expression (P=0.0023). Knockdown of miR-3127-5p significantly inhibited HCCLM3 cell migration and invasion compared with negative controls. Cyclin-dependent kinase 10 (Cdk10), the potential downstream of miR-3127-5p, was validated by luciferase reporter assay. Rescue assay showed that Cdk10 mediated the effects of miR-3127-5p on migration and invasion of HCC cells. Conclusion: miR-3127-5p is upregulated in HCC, which promotes HCC cell migration and invasion through targeting Cdk10.