In vitro and in vivo studies on the effect of inhibiting the expression of CIP2A on the biological characteristics of HepG2 cells / 西安交通大学学报(医学版)

ABSTRACT

Objective: To investigate the effect of inhibiting CIP2A expression on the biological characteristics of HepG2 cells. Methods: The recombinant adeno-associated viral vector rAAV2-EGFP-CIP2A shRNA was successfully transfected into HepG2 cell lines by using previous experiments. Cell proliferation was detected by MTT assay, cell apoptosis was measured by flow cytometry, and cell invasion ability was detected by Transwell assay. A model of subcutaneous implant tumor in nude mice was established to observe the growth of xenograft tumor in nude mice. The volume and quality of tumor growth were detected in nude mice after 30 days, and the expression of CIP2A protein in the transplanted tumor tissues was detected by immunohistochemistry. Results: The results of MTT and flow cytometry showed that the proliferation and apoptosis of HepG2 cells were inhibited after downregulation of CIP2A expression. The Transwell invasion experiment indicated a decrease in cell invasion ability. The result of subcutaneously growing tumor in the nude mice found in the interference experiment group of nude mice, the subcutaneous tumor growth rate, the final volume and weight were much smaller than those in blank control group and no-load virus group, CIP2A protein expression in transplanted tumor tissues was significantly lower. Conclusion: CIP2A promotes the proliferation, invasion of HepG2 cells and malignant growth in vivo.