BUB1 expression is enriched in glioblastoma and promotes tumor proliferationin glioblastoma / 西安交通大学学报(医学版)

ABSTRACT

Objective: To investigate the functions of mitosiskinase budding uninhibited by benzimidazoles 1 (BUB1) for cell proliferation in glioblastoma (GBM). Methods: We investigated the expression level of BUB1 in GBM cells by analyzing TCGA database. Then Real-time PCR and Western blot were used to explore the role of BUB1 in proliferation of GBM cells via BUB1 silencing using siRNA transfection. Additionally, we investigated the correlation between BUB1 expression and prognosis of GBM patients. Results: BUB1 expression was significantly elevated in GBM patients, while artificial silencing of BUB1 attenuated the proliferation (P=0.007) and cloning formation of U87 cells (P=0.033). At the same time, improved prognosis could be observed in GBM patients with highly expressed BUB1 compared with those with lower BUB1 expression (P=0.040). Conclusion: Increased BUB1 expression promotes the growth and proliferation of GBM cells and is correlated with the poor prognosis of GBM patients.