Effects of puerarin on the expressions of CaM, CaMKⅡ, MECP2, BDNF and Akt in oxygen and glucose deprivation cell models / 西安交通大学学报(医学版)

ABSTRACT

Objective: To observe the effects of puerarin on the expressions of CaM, CaMKⅡ, BDNF and Akt in vascular dementia cell models induced by oxygen and glucose deprivation (OGD). Methods: The passaged well-differentiated PC12 cells were randomly divided into control group, model group, and low-dose, medium-dose and high-dose intervention groups. Vascular dementia cell model was established by OGD. Suitable OGD time and concentration of puererin were obtained from the cell viability measured by MTT assay. The release of LDH was measured to assess the extent of cell damage and identify cell models. The expressions of CaM, CaMKⅡ, MECP2, BDNF and Akt were detected by Western blot. Results: PC12 cells with OGD prolonged viability decreased in a time-dependent manner, with increased concentrations of puerarin increased in a concentration-dependent manner. Effective intervention of puerarin was 0.1-10 μmol/L and optimal time of OGD was 6 h. Compared with control group, the release of LDH in model group was significantly increased (P0.05). Puerarin could down-regulate the level of CaM protein, increase the expressions of MECP2 and BDNF and the phosphorylation of CaMKⅡ, and also increase the phosphorylation of Akt in addition to the low-dose group (P<0.05). Conclusion: The neuroprotective effect of puerarin may be related to the increase of the autophosphorylation of CaMKⅡ mediated by Ca2+-CaM complex, induce the phosphorylation of MECP2, up-regulate the expression of BDNF and activate the PI3K-Akt pathway to inhibit the expression of apoptotic genes and proteins.