Preparation and histological evaluation of human decellularized adipose tissue / 解剖学报

ABSTRACT

Objective To decellularize adipose tissue derived from human liposuction, evaluate the histological composition of human decellularized adipose tissue, and explore the possibility for it as tissue engineering scaffold materials. Methods The adipose tissue derived from five human liposuction was decellularized by a series of physical chemistry and enzymology method . The general morphology of the decellularization process was observed. HE staining, Masson staining, Gomori staining, oil red 0 staining and DAPI staining were performed and the content of DNA, collagens and glyeosaminoglycans remaining in the decellularized adipose tissue were quantitatively detected. Results The decellularized adipose tissue was some kind of milky, soft, variable insoluble material. HE staining and DAPI staining showed no residual cell components. Masson staining showed the presence of collagen fibers. Gomori staining showed the presence of reticular fibers, and oil red 0 staining showed no visible lipid residues. The residual DNA content in the tissue is very tiny and the collagen and glycosaminoglycan contents were retained. Conclusion The adipose tissue derived from the body' s liposuction can be decellularized by physical, chemical, and enzymatic method . The cells and lipid in the decellularized adipose tissue can be effectively removed, and the components of the extracellular matrix of the adipose tissues are mostly retained.