Novel protein C7orf42 promotes rat hepatocyte BRL-3A proliferation / 解剖学报

ABSTRACT

Objective: To explore the effect of C7orf42 on cell proliferation of rat hepatocyte line BRL-3A in vitro. Methods: The expression of C7orf42 was knocked down by siRNA, and MTT and EdU assay were used to discover the effect of C7orf42 on cell proliferation at 48 hours after transfection. Flow cytometry was used to observe the effect of cell cycle progression. Real-tme PCR and Western blotting were used to detect the changes in the expression of cell proliferation-associated gene. Results: MTT results showed that the cell viability of the interference group (C7BRL-siR3) was significantly lower than that of the negative control group (NC) at 48 hours after transfection (P < 0. 05). Meanwhile, the percentage of EdU-labeling cells was also significantly decreased (P < 0. 05). At the same time, the flow cytometry results showed that the number of cells in division phase (S + G2/M) of the interference group was significantly reduced in parallel (P < 0. 05). Further, the interference group down-regulated the expression levels of cell proliferation-related genes and proteins of JUN, MYC, CCND1 and CCNA2. Conclusion: C7orf42 may promote cell proliferation via regulating the expression of JUN, MYC, CCND1 and CCNA2 in rat hepatocyte line BRL-3A.