Effects of resveratrol pretreatment on neurite growth of primary cortical neurons after oxygen-glucose deprivation/reperfusion injury in rats / 解剖学报

ABSTRACT

Objective To investigate the effects of resveratrol pretreatment on neurite growth of rat primary cortical neurons after oxygen-glucose deprivation/reperfusion (OGD/R) injury in vitro. Methods Primary cortical neurons were cultured under oxygen and glucose deprivation for 150 minutes and reoxygenation for 24 hours. The study had the normal, control and 5μmol/L resveratrol pretreatment groups. Neurons were identified with immunofluorescence. Cell viability was detected with cell counting kit-8(CCK-8) assay. Cell apoptosis was detected with TUNEL assay. Immunofluorescence and Western blotting measured the expressions of microtubule-associated protein 2(MAP-2) and growth associated protein 43 (GAP-43), and the length and number of neurites were counted. Results Cells had high expression of neuronal specific marker MAP-2. Compared with the control group, resveratrol treatment significantly enhanced the neurons viability (0.551±0.009 vs 0.436±0.013, P<0.01), decreased the numbers of apoptosis (18.3% ±1.3% vs 35.3% ±1.9%, P<0.01), upregulated the expressions of MAP-2 (0.790 ± 0.102 vs 0.462 ±0.063, P <0.01) and GAP-43 (0.768 ± 0.084 vs 0.424 ±0.065, P< 0.01) proteins, increased the length (89.510 ± 6.939 vs 61.538 ± 9.14, P < 0.01) and numbers (6.347 ± 1.002 vs 3.040 ± 0.608, P < 0.01) of neurites. Conclusion Resveratrol pretreatment can reduce injury and promote neurite growth of cultured neurons after OGD/R.