Expression and alteration induced by MPTP/MPP+ of mitochondrial transcription termination factors family / 解剖学报

ABSTRACT

Objective To investigate the expression of mitochondrial transcription termination factors (MTERFs) in various tissues and cells, and the changes of MTERFs induced by l-methyl-4-phenyl-l, 2, 3, 6-tetrahydropyridine (MPTP)/l-methyl-4-pheny lpyridine (MPP+). Methods Western blotting was used to measure the expression of the MTERFs in brain, liver, kidney of C57BL mice and in human SH-SY5Y, HCM, L-02 cells. MPTP/MPP+ was injected to imitate PD animal/cell models. The changes of MTERFs protein were detected through western blotting. Results The expression of MTERFs was higher in mice brain and SH-SY5Y cells. In PD mouse model induced by MPTP, MTERF3 reduced significantly. Conclusion The expression of MTERFs was higher in mouse brain and SH-SY5Y cells. After induced by MPTP/MPP+, MTERF3 decreased obviously, which increased transcription level and might involve in a process of Parkinsons disease.