Determination of enantiomeric impurity of etomidate by high performance liquid chromatography / 西安交通大学学报·英文版
Academic Journal of Xi'
;
an Jiaotong University;(4): 102-104, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-844732
ABSTRACT
Objective:
To determine enantiomeric impurity of etomidate using high performance liquid chromatography.Methods:
(R)-etomidate and (S)-etomidate were separated on a CHIRALPAK AD-H column. The mobile phase consisted of 20 80(v/v) isopropanol-n-hexane. The flow rate of the mobile phase was 0.5 mL/min. The detected wavelength was 242 nm.Results:
(R)-etomidate and (S)-etomidate could be separated completely under these conditions. The precision of (R)-etomidate was 1.57% (n = 3). The limit of detection of (R)-etomidate was 4. 25 ng/mL. The average percentage content of (S)-etomidate was 0.09% in the samples.Conclusion:
The method was repeatable and sufficiently sensitive to determine the enantiomeric impurity of etomidate. It allows the quantitation of the impurities at the 0.085% (w/w) level relative to etomidate at a concentration of the test solution of 5 mg/mL.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Xi'an Jiaotong University
Year:
2010
Type:
Article
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