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Luminol-potassium permanganate chemiluminescence system for the determination of three anthracycline antibiotics / 西安交通大学学报·英文版
Academic Journal of Xi&#39 ; an Jiaotong University;(4): 20-24, 2010.
Article in Chinese | WPRIM | ID: wpr-844745
ABSTRACT

Objective:

To establish a flow-injection chemiluminescence method for the determination of doxorubicin, epirubicin and mitoxantrone and study its reaction mechanism.

Methods:

In alkaline medium, chemiluminescence of luminol-potassium permanganate system could be inhibited obviously by anthracycline antibiotics. Combined with flow-injection technique, a new chemiluminescence method for determining the anthracycline antibiotics was set up. The chemiluminescence mechanism of the luminol-potassium permanganate system was also discussed.

Results:

Under optimal conditions, the good linear ranges of doxorubicin, epirubicin and mitoxantrone were 5.0×10-9-1.0×10 -6 g/mL, 1.0×10-9-1.0×10-5 g/mL and 3×10-9-1.0×10-6 g/mL, respectively. The detection limits of doxorubicin, epirubicin and mitoxantrone were 3.0×10-9 g/mL, 5.0×10-6 g/mL and 2.0×10-9 g/mL, respectively. During eleven repeated inter-day and intra-day precision tests of 1.0×10-6 g/mL samples, the relative standard deviations corresponded to reference values of 3.0% ,2.8% and 2.1%.

Conclusion:

The developed method is sensitive, accurate, rapid and of low cost. It can be applied to determine doxorubicin hydrochloride, epirubicin hydrochloride and mitoxantrone hydrochloride in injection preparations.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Academic Journal of Xi'an Jiaotong University Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Academic Journal of Xi'an Jiaotong University Year: 2010 Type: Article