Asymmetric PCR method in generation of HBV ssDNA for pyrosequencing / 西安交通大学学报·英文版
Academic Journal of Xi'
;
an Jiaotong University;(4): 54-56, 2009.
Article
in Chinese
| WPRIM
| ID: wpr-844803
ABSTRACT
Objective:
To explore the optimal primer ratio and concentration of asymmetric polymerase chain reaction (A-PCR) in producing hepatitis B virus (HBV) single-stranded DNA (ssDNA) for pyrosequencing.Methods:
A-PCR was carried out to generate HBV ssDNA with forward to reverse primers of different ratios (501, 1001) and concentrations (13.0 pmol/25μL and 0.14 pmol/25μL, 19.5 pmol/25μL and 0.21 pmol/25μL), and the product yield and quality were compared respectively.Results:
The forward to reverse primer ratio of 501 provided better yield and concentration of 19.5 pmol/25μL and 0.21 pmol//25μL generated a clearer band.Conclusion:
A simple and feasible method to produce HBV ssDNA for pyrosequencing in batch is established.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Xi'an Jiaotong University
Year:
2009
Type:
Article
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