Identification of differentially expressed genes in two new human bladder carcinoma cell lines / 西安交通大学学报·英文版
Academic Journal of Xi'
;
an Jiaotong University;(4): 182-185, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-844858
ABSTRACT
Objective:
To screen and identify differentially expressed genes in two new human urothelial carcinoma cell lines, BLS-211 and BLX.Methods:
Suppression subtractive hybridization (SSH) was used to createa subtracted library, and clones were sequenced.Results:
Totally 13 over-expressed genes in BLX and 9 in BLS-211 cells were obtained, respectively. Among them, 18 were known genes and 4 were new ESTs (Expressed Sequence Tag), and were collected by GenBank dbEST database (The access number was EB390424-7).Conclusion:
SSH is a powerful method for the identification of differentially expressed genes. The differential expression of some BCG-associated genes in different cells may be related to the different responses to clinical BCG therapy. The identified new ESTs can be cloned for full length to further study their functions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Academic Journal of Xi'an Jiaotong University
Year:
2007
Type:
Article
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