Study on differentiation of rats embryonic stem cells cultured in BRL-CM into neural precursor cells / 西安交通大学学报·英文版
Academic Journal of Xi'
;
an Jiaotong University;(4): 55-58, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-845118
ABSTRACT
Objective:
To investigate whether buffalo rat liver cell-conditioned medium (BRL-CM) can be used as the culture medium of embryonic stem (ES) cells, and to get relatively pure neural precursor cells (NPCs) for treatment aim.Methods:
Mouse ES cells were cultured in BRL-CM and medium contain leukemia inhibitory factor (LIF), respectively. NPCs were selectively cultured in serum-free medium. Alkaline phosphatase activity was visualized with NBT/BCIP and nestin antigen was detected with immunocytochemical methods.Results:
BRL-CM could be used as an efficiency culture condition instead of LIF in ES cells culture. About 86% of cells derived from ES cells in the serum-free culture were NPCs.Conclusion:
BRL-CM can replace LIF to use in ES cell culture. High purity of NPC can be induced from ES cells with serum-free culture method.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Xi'an Jiaotong University
Year:
2003
Type:
Article
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