Protective effect of the Diaphragma Juglandis Fructus extracts on the rhabdomyolysis-induced acute kidney injury in rats / 国际药学研究杂志

ABSTRACT

Objective: To investigate the protective effect and possible mechanism of Diaphragma Juglandis Fructus(DJF) extracts on the rhabdomyolysis-induced acute kidney injury(AKI)in Sprague-Dawley(SD)rats. Methods: Sixty male SD rats were randomly divided into 6 groups normal control group, model group as well as the four test groups treated with 180 mg/kg and 360 mg/ kg water extract or alcohol extract of DJF. The rats were orally administered once a day by gavage with the designed concentration of test substances or distilled water(for controls)for 30 days. After the last drug administration, all rats except for those in the normal control group were subjected to the intramuscular injection of 50% glycerol to both hind limbs to create the rat model of rhabdomyolysis-induced AKI. The renal function and kidney histopathology were then examined and the serum level of tumor necrosis factor-alpha (TNF-ɑ)and antioxidant indicators were detected at 24 and 48 hours after the glycerol injection. Results: Compared with the normal control group, the urine volume for 24 h as well as the serum glutathione peroxidase(GSH-Px)and total superoxide dismutase(TSOD)activities were significantly decreased, while the serum creatinine(Cr), blood urea nitrogen(BUN), TNF-ɑ and malondialdehyde(MDA)level was significantly increased in the model group(P0.05). However, the intervention with the 360 mg/kg DJF water extract significantly increased the urine volume for 24 h, enhanced the serum T-SOD activity on the 48 h, and decreased the serum Cr, BUN, MDA and TNF-α level on the 48 h(P<0.05 or P<0.01). Conclusions: Both the 360 mg/kg DJF alcohol and water extracts could effectively protect the rhabdomyolysis-induced AKI, which caused by glycerol in SD rats, and the effect of the alcohol extract was better than that of the water extract. The possible mechanism is likely related to the inhibition of inflammation and a positive role in antioxidation.