Protective effect of the Potentilla anserina polysaccharide on high altitude cerebral edema and high altitude pulmonary edema in rats / 国际药学研究杂志

ABSTRACT

Objective: To test the anti-plateau hypoxia effect of Potentilla anserina polysaccharide(PAP), Cynomorium songarieum Rupr. polysaccharide(SCRP), Sphallerocarpus gracilis polysaccharide(SGP)and Lilium brownii polysaccharide(LP), and then investigate the protective effect of most effective polysaccharide on the high altitude cerebral edema(HACE)and high altitude pulmonary edema(HAPE)in rats. Methods: PAP, CSRP, SGP and LP were prepared by the water extraction-alcohol precipitation method. The normobaric hypoxia test and acute hypoxia test were performed to find out the polysaccharide with the best anti-hypoxia effect and the related dose-dependent effect in mice. A large hypobaric hypoxia chamber stimulating 8000 m altitude was used to investigate pathological changes and water contents in rat brain and lung tissue before and after hypoxia, and the concerned oxidative stress and inflammation related parameters were also measured. The protective effect of PAP on the high altitude cerebral and pulmonary edema was evaluated by the rat model experiments. Results: PAP showed the best and dose-dependent anti-hypoxia effect among the four polysaccharides. The water content in brain and lung tissues of rats increased obviously in the hypoxia model(HM)group, and the brain tissue cell hierarchical fuzzy, lung tissue congestion and edema as well as the heavy inflammatory cell infiltration, widening of alveolar interval and thickening of alveolar wall were also found in the HM group. The enzymatic activity of SOD was notablely depressed, while both the MDA and IL-1β contents in brain and lung tissues remarkably increased(P<0.01)in the HM group. Com- pared with the HM group, the water content in the rat brain and lung tissues significantly decreased in each of the different dose PAP groups(P<0.05 or P<0.01), and the inflammatory cell infiltration, alveolar interval, and thickness of alveolar wall all notabely decreased in the HM group. Furthermore, the enzymatic activity of SOD increased, while both the MDA and IL-1β contents decreased, all significantly in the PAP groups than in the HM group(P<0.05 or P<0.01). Conclusion: PAP showed a good anti-hypoxia effect and effectively inhibited HACE and HAPE to exert a certain protective effect in a rat model.