Simultaneous determination of baicalein and wogonin in Baipuhuang tablets by HPLC / 国际药学研究杂志
Journal of International Pharmaceutical Research
;
(6): 63-66, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-845216
ABSTRACT
Objective:
To establish an HPLC method for simultaneous determination of baicalein and wogonin in Baipuhuang tablets.Methods:
HPLC was performed on an Agilent C 18 column(4.6 mm×250 mm, 5 μm)using the gradient acetoni trile(A)-water(B)at the 1.0 ml/min flow rate as mobile phase. The columm temperature was 30℃ and the detection wavelength was 280 nm.Results:
The linear regression equation of baicalein was Y=24117X-223.95(r=0.9990);the linear range was 0.02~0.2 μg with the average recovery of 98.95% and the RSD of 0.64%(n=9). The linear regression equation of wogonin was Y=16237X-26.698(r=0.9996);the linear range was 0.02~0.2 μg with the average recovery of 99.28% and the RSD of 1.03%(n=9). The RSD values for the precision, stability and repeatability were all less than 2%.Conclusion:
The established HPLC method appears to be highly sen sitive, accurate and reproducible, which could be used for the quality control of Baipuhuang tablets.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of International Pharmaceutical Research
Year:
2020
Type:
Article
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