Preparation and in vitro antitumor activity of curcumin micelle / 国际药学研究杂志

ABSTRACT

Objective: To prepare curcumin(Cur)micelles with D-α-tocopherol polyethylene glycol 1000 suc- cinate(TPGS)as carrier, so as to improve the water solubility and antitumor activity of curcumin. Methods: The prepa- ration method of Cur/TPGS micelles was investigated under the indication of particle size and drug loading of the micelles. The particle size of micelle and Zeta potential were used as evaluation indices to optimize the feed ratio of Cur/TPGS. The particle size and morphology of the micelles were characterized by the dynamic light scatter(DLS)method and transmis- sion electron microscopy(TEM). The in vitro antitumor activity of Cur and Cur/TPGS micelles was evaluated by the MTT method. Results: The Cur/TPGS micelle was manufactured by the dialysis method. The optimum feed ratio of Cur/TPGS was 110. The Cur/TPGS micelles showed a spherical shape, with the average particle size and Zeta potential of 54.4 nm and-12.1mV, respectively. The drug loading and the encapsulation efficiency were 7.35% and 81.3%, respectively. The Cur/TPGS micelles had a good stability and showed an in vitro antitumor activity better than Cur in the MTT test. Conclu- sion A stable Cur/TPGS micelle was prepared using the dialysis method in the present study, which greatly improved the water solubility and in vitro antitumor efficacy of Cur.