Simultaneous determination of four effective components in Taraxaci Herba by UPLC-MS/MS / 国际药学研究杂志

ABSTRACT

Objective: To establish an LCMS/MS method for simultaneous determination of pcoumaric acid, caffeic acid,luteolin and luteoloside in Taraxaci Herba. Methods: The LC analysis was performed on a UPLC-MS/MS instrument with a Dikma Endeavorsil UPLC C18 column(100mm×2.1 mm,1.8 μm),the mobile phase was 0.1% formic acid solution-methanol in a gradient elution,and the flow rate was 0.2 ml/min;the column temperature was set at 35℃; the injection volume was 2 μl. For the MS/MS analysis,the ESI source was operated in the positive-ion mode and the ions were monitored in multiple reaction monitoring(MRM)mode,the temperature of drying gas was 370℃,the sheath gas pressure was set at 10 Bar,the aux gas pressure was maintained at 30 Bar,and the spray voltage was controlled at 4000 V. Results Calibration curves showed good linearity for all 4 compounds in the concentration range of 0.1-10 μg/ml,and the correlation coefficient®was 0.9991,0.9996,0.9994 and 0.9999 for p-coumaric acid,caffeic acid,luteolin,and luteoloside,respectively. Conclusion: The present method could be used for the simultaneous determination of the above mentioned 4 components in Taraxaci Herba.