Preparation, characterization and biological test of biodegradable composite tissue engineering scaffold / 国际药学研究杂志

ABSTRACT

We report fabrication of electro-spun polycaprolactone (PCL), gelatin and PCL–gelatin scaffolds in tissue engineering based applications. The structural characterizations were investigated by fourier transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) for the fabricated scaffolds. Pure PCL and gelatin scaffolds showed 3D nano−fibrous porous structures at 20 wt. % and 30 wt. %, respectively. PCL−gelatin scaffold at 7030 vol. % showed good fibrous morphology without any structural discontinuities such as polymer beads, droplet formation and uneven fibers. Cellular activities such as cell adhesion and growth on scaffold surfaces were performed with F11 human fibroblast cells via MTT assay. Cell viability study showed that PCL−gelatin scaffold significantly improved the cell adhesion and proliferation when compared to pure gelatin and PCL scaffolds. Thus, the PCL−gelatin scaffold reported in our work has major prospective in the area of wound dressings, treatment of diabetic foot ulcers, implants and transdermal drug delivery.