LC-Ms/Ms method developed for determination of albiflorin in rat plasma and its application on toxicokinetic study / 国际药学研究杂志

ABSTRACT

Objective To develop and validate a LC-MS/MS method for the determination of albiflorin in rat plasma. Methods Plasma samples were extracted by liquid-liquid extraction with a mixture of ethyl acetate-isopropanol (955, V/V) to prepare samples for analysis. Chromatographic separation was performed on a Poroshell 120 EC-C18 column (50 mm× 2.1 mm I.D. 2.7 µm). The mobile phase consisted of acetonitrile-water followed gradient elution. Detection of albiflorin and the internal standard (IS) lacosamide was achieved by ESI MS/MS in the positive ion mode using m/z 498.5→m/z 197.1 and m/z 251.3→m/z 108.2 transitions, respectively. Results The method was linear in the range of 20 to 2000 ng/ml when 50 µl plasma was analyzed. The lower limit of quantification was 20 ng/ml. The inter- and intra-day precision values were both below 15%, and the accuracy (relative error) was within ±8.06% in all quality control samples. Conclusion The method provides a sensitive and rapid means for the determination of albiflorin in rat plasma, and completely meets the requirements for toxicokinetic study.