Detection and identification of high producing huperzine A by an endophytic fungus from Huperzia serrata / 国际药学研究杂志

ABSTRACT

Objective To screen endophytic fungal strains isolated from wild Huperzia serrata which can highly produce huperzine A. Methods The strain producing huperzine A was identified through thin layer chromatography (TLC) and high- performance liquid chromatography(HPLC) with authentic huperzine A. The fungus was identified according to its morphological characteristics and nuclear ribosomal DNA ITS sequence. Results The strain was identified as Fusarium oxysporum NSG-1 according to its morphological characteristics and nuclear ribosomal DNA ITS sequence. The amount of huperzine A produced by the fermentation liquor of this endophytic fungus was quantified to be 1.11 mg/100 ml by HPLC, which was higher than that of previously reported endophytic fungi. Conclusion Endophytic fungus producing high huperzine A can be obtained from H. serrata. The production of huperzine A based on mutagenesis and transformation of the obtained strain may adapt to the industry production.