Effect of alcohol extract of Cortex Eucommiae on oxidative stress markers in serum of brain aging model mice / 国际药学研究杂志

ABSTRACT

Objective To explore the effect of Cortex Eucommiae (CE) extract on oxidative stress markers of brain aging mice. Methods Brain aging mice model was made via subcutaneously administered D-galactose. The ethanol extract of CE was intra-gastrically administered to the model mice. Apoptosis ratio of brain cells of mice was determined by a flow cytometry to evaluate the anti-aging function of CE extract. A series of biomarkers related to oxidation, including malonyldialdehyde (MDA), superoxide dis-mutase (SOD), protein carbonyl (PCO), and nitric oxide (NO) in serum of mice were determined by ELISA. Results Compared with the model mice, the apoptosis ratio of brain cells in the CE extract group decreased significantly (P<0.01). ELISA test results showed that,compared with the normal group, NO and SOD levels in serum of CE extract group were significantly higher (P<0.01). Compared with the model group, MDA and PCO levels in serum of CE extract group were significantly lower (P<0.01), and SOD level was obviously higher (P<0.01). Conclusion The CE extract might play the role of brain anti-aging by the effective attenuation of oxidative damage.