Chemiluminescent immunoassay method for determination of cathelicidin-BF in rat serum / 国际药学研究杂志

ABSTRACT

Objective To establish a chemiluminescent immunoassay method for the determination of cathelicidin-BF (BF-30) in rat serum, to provide a fast and simple analytical method for its pharmacokinetic studies, then to investigate the pharmacokinetic characteristics of BF-30 in vivo. Methods A chemiluminescent immunoassay method was developed and the matrix effect, precision, accuracy, stability and the dilution effect were evaluated, then the method was used to determine the concentration of BF-30 in rat serum. Results The linear range of BF-30 in rat serum was 0.5-40 ng/ml, and the lowest limit of determination was 0.5 ng/ml. No matrix effect was observed. The RSD of inter-and intra-day precisions were 8.76%-14.15% and 4.91%-11.11%, respectively, and accuracy was-1.27%--7.71%. The stability of samples for 13 days and stock solution for 30 days at-20°C were good, and the stability of serum samples after 2 cycles of freeze-thaw met the requirements. There was no dilution effect noted after 40 times sample dilution. Conclusion We heve developed a simple, accurate and reliable method which can be applied to the determination of BF-30 in rat serum and following pharmacokinetic study.