Effect of estrogen on IL-10 production in mouse splenic B cells in vitro / 国际药学研究杂志

ABSTRACT

Objective To ascertain whether estrogen could induce B cell to produce interleukin (IL) -10. Methods C57BL/ 6 splenic B cells were purified by magnetic activated cell sorting (MACS) method, followed by estrogen treatment for 3 days. The secretion of IL- 10 from cultured cell supernatant was tested by ELISA technique. The abundance of mRNA for IL- 10, PD- L1 and RBM47 in B cells with estrogen treatment was tested by real-time PCR method. The intracellular IL-10 expression and the surface PD-L1 expression of treated B cells were tested by fluorescence activated cell sorting (FACS) method. And the expression of RBM47 in B cells by estrogen treatment was tested using Western blotting method. Results Estrogen could induce B cell to produce IL- 10 in a dose-dependent manner. Estrogen treatment could increase the percentage of IL-10+ B cells, the abundance of mRNA for IL-10, PD-L1 and RBM47 in B cells, as well as the expression of PD-L1 on B cell surface. Furthermore, our experimental result indicated the upregulation of RBM47 expression in B cells by estrogen treatment. Conclusion Estrogen treatment in vitro can induce the upregulation of IL- 10+ regulatory B cells (Breg). Upregulation of RBM47 in the treated B cells might participate in this process by stabilizing IL- 10 mRNA.