Pharmacodynamic study of Sophora flavescens alkaloid gels for treatment of vaginal candidiasis / 国际药学研究杂志

ABSTRACT

Objective To explore the pharmacodynamic effect of Sophora flavescens alkaloid (SFA)gels for the treatment of vaginal candidiasis on animal models. Method The BALB/c mice models having vaginal candidiasis were prepared. The animals were divided into four groups the model group, the blank gel group, the SFA gel group, and the ketoconazole cream group. The regimens were administered to the animals once a day for seven days. Before treatment and after the fourth and seventh treatments, the vaginal smears were collected and Gram-stained. The vaginal washings were cultured to detect Candida albicans. On day 7, the mice were sacrificed and the vaginal tissues were excised for pathological detection. Results Compared with the model and the blank gel groups, the SFA gel and ketoconazole cream groups reduced the C. albicans colonies in the vagina. Moreover, the SFA gels increased the number of Lactobacillus, the helpful bacteria in vagina but the ketoconazole creams did not. The SFA gels and ketoconazole creams inhibited the growth of C. albicans according to the investigation of in vitro culture of vaginal washings (P<0.01). Furthermore, the SFA gels inhibited the inflammatory hyperplasia and edema. Conclusion The therapeutic action of SFA gels on vaginal candidiasis is achieved by inhibiting the growth of candidiasis, promoting lactobacillus growth and inhibiting the inflammatory hyperplasia.