Chromatographic fingerprint peaks matching of gualou xiebai baijiu decoction and its medicinal ingredients in prescription by evolving window orthogonal projection analytical method / 国际药学研究杂志

ABSTRACT

Objective To explore whether evolving window orthogonal projection analytical method can be used in the chromatographic peaks matching between traditional Chinese medicine preparation and its medicinal ingredients in prescription. Methods The fingerprints of Gualou Xiebai Baijiu Decoction and the herbs (Gualou and Xiebai) in its prescription were established by using high performance liquid chromatography-photodiode array detector (HPLC-PDAD), and the HPLC peaks in fingerprints of Gualou Xiebai Baijiu Decoction and the herbs in its prescription were matched according to the principle of evolving window orthogonal projection analytical method. Results Evolving window orthogonal projection analytical method could match the chromatogram peaks well between different fingerprints of Gualou Xiebai Baijiu Decoction and the herbs in its prescription. There were 20 common peaks between the ethyl acetate extraction of Gualou Xiebai Baijiu Decoction and Gualou, and 9 common peaks between the n-butanol extraction of Gualou Xiebai Baijiu Decoction and Xiebai. Conclusion Evolving window orthogonal projection analytical method can be used in the chromatographic peaks matching between traditional Chinese medicine preparation and its medicinal ingredients in prescription.