Residues determination of oxytetracycline, tetrarcycline and chlortetracycline in zaosheng cattle meat by SPE-HPLC / 国际药学研究杂志

ABSTRACT

Objective To establish a method for simultaneous determination of 3 tetracyline antibiotics (TCs) oxytetra-cycline (OTC), tetracycline (TC), chlorotetecycline (CTC)) residues in Zaosheng cattle meat using solid phase extraction-high performance liquid chromatography (SPE-HPLC). Methods The samples were extracted by Na2EDTA-Mcllvaine buffer solution (pH=4.0), and 15% trichloroacetic acid (TCA) as the protein precipitation solution, then the targets were cleaned up and enriched by C18 solid phase extraction column. HPLC Mobile phase consisted of oxalic acid (0.01mol/L), acetonitrile as well as methanol. The detection wavelength was 350 nm and external standard method was adopted for quantification. Results The calibration curves of 3 TCs had good linearity with correlation coefficients of 0.998. The limits of detection of OTC, TC and CTC were 18, 15, 30 μg/kg, respectively, while their average recoveries were 76.10%-95.22%, with relative standard deviations (RSD) lower than 10% after optimized pretreatment and analytical conditions. Conclusion The method has high sensitivity, precision, and good repeatability, and can meet the needs of residue determination of TCs in meat.