Acute oral toxicity and genotoxicity of Rosa laxa Retz. var. mollis Yu et Ku cynarrhodion extracts / 国际药学研究杂志

ABSTRACT

Objective To study the acute oral toxicity and genotoxicity of Rosa laxa Retz. var. mollis Yu et Ku cynarrhodion extracts. Methods The acute oral toxicity in mice and rats (maximal tolerance dose, MTD) were observed for 14 days after administration. Genetictoxicity was evaluated on Salmonella typhimurium reverse mutation assay (Ames test), in vivo mammalian erythrocyte micronucleus assay, sperm malformation assay in mice and single cell gel electrophoresis (Comet assay). Results In acute oral toxicity study, the behavior, clinical signs and body changes were normal in all experimental animals. LD50 > 15.0 g/kg (rats) and 20.0 g/kg (mice). In the test dose range, results of the four genotoxicity tests were negative. Conclusions In accordance with internationally recognized animal ethics and technical specifications for the experiments using SPF animals in our lab, the acute oral toxicity of R. laxa Retz. var. mollis Yu et Ku cynarrhodion extracts is classified as nontoxic, and genotoxicity is not observed under the test condition.