Determination of propylene glycol content in recombinant human glucagon-like peptide-1 (7-36) injection by GC / 国际药学研究杂志
Journal of International Pharmaceutical Research
;
(6): 229-231, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-845684
ABSTRACT
Objective:
To establish a method to detect the content of propylene glycol in the recombinant human glucagon-like peptide-1 (rhGLP-1) (7-36) injection by gas chromatography (GC).Methods:
GC was performed using a quartz capillary column (30 m×320 μm,0.5 μm) with the mobile phase of N2 and inlet temperature of 230℃ at the flow rate of 1ml/min and column temperature of 250 ℃, the injection volume was 1μl.Results:
The calibration curve was linear within the range of 0.5-1.5 mg/ml, r=0.9991. The average recovery rate was 100.2%; under different chromatographic conditions, the method had good stability, RSD was less than 2.0%.Conclusion:
This method is simple, accurate and reproducible, and can be applied in the determination of propylene glycol content in rhGLP-1 (7-36) injection.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of International Pharmaceutical Research
Year:
2015
Type:
Article
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