Procyanidin inhibits multidruy resistance 1 mRNA expression via regulating microRNA-27a and microRNA-451 / 国际药学研究杂志
Journal of International Pharmaceutical Research
; (6): 501-506, 2015.
Article
in Zh
| WPRIM
| ID: wpr-845718
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WPRO
ABSTRACT
Objective To investigate the effect of expression of microRNA-27a(miR-27a) and microRNA-451 (miR-451) in A2780/T cells and its relativity to multidrug resistance (MDR); mRNA inhibition by procyanidin. Methods Stem-loop PCR method was performed to evaluate the expression of miR-27a and miR-451 in use of procyanidin (0-40 mmol/L) in 0-48 h in A2780/T cells. Additionally, over-expressing or interfecting microRNAs by using mimics or inhibitor of miR-27a and miR-451, the expression of MDR1 mRNA was assessed by RT-PCR in cells exposing to procyanidin. Results The expression of miR-27a and miR-451 was significant inhibited by procyanidin in both time- and concentration-dependency. Over-expressed MDR1 mRNA associated with miR- 27a or miR-451 mimics was blocked by procyanidin, whereas there was no effect on down-expressed MDR1 mRNA associated with miR-27a or miR-451 inhibitor by procyanidin. Conclusion Procyanidin inhibits MDR1 mRNA expression by inhibiting miR-27a and miR-451 expression in A2780/T cells.
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Language:
Zh
Journal:
Journal of International Pharmaceutical Research
Year:
2015
Type:
Article