Flow injection chemiluminescence determination of biapenem for injection / 国际药学研究杂志

ABSTRACT

Objective To establish a new method for the rapid and sensitive determination of biapenem. Methods A simple, rapid and sensitive flow injection chemiluminescence(CL) method for the determination of biapenem was developed based on the fact that biapenem could enhance the CL of the luminol (LMN)-potassium ferricyanide reaction system under an alkaline condition. Results Under the optimal experimental conditions, we obtained a linear relationship between the CL intensity and the concentration of biapenem in the range of 0.02%0.6 mg/L with a detection limit of 7.3 x10-3 mg/L. The relative standard deviation was 1.76% for the eleven-time determination of 0.2 mg/L biapenem. Conclusion The method shows high sensitivity, low detection limit and good repeatability. It has been successfully applied to the determination of biapenem for injection and the results are satisfactory.